κ receptor - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Homo sapiens

GPCR PREFERRED CHAIN

F

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

22EM

RESOLUTION

2.9 Å

REFERENCE

PUBLICATION DATE

May 6, 2026

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

52 interactions (Click to see)

LIGANDS

beta01 - Agonist

PHYSIOLOGICAL LIGANDS

[Met]enkephalin (Human), β-neoendorphin (Human), dynorphin A (Human), dynorphin A-(1-8) (Human), dynorphin A-(1-13) (Human), big dynorphin (Human), β-endorphin (Mouse), β-endorphin (Rat), dynorphin B (Human), β-endorphin (Human), α-neoendorphin (Human), [Leu]enkephalin (Human)



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Hydrophobic ICL2 P 163 34x50 G.H5 I 343 G.H5.15
Van-der-waals ICL2 P 163 34x50 G.H5 I 343 G.H5.15
Hydrophobic TM6 N 268 6x29 G.H5 F 354 G.H5.26
Hydrophobic TM5 L 253 5x65 G.H5 I 344 G.H5.16
Hydrophobic ICL3 L 259 - G.H5 L 348 G.H5.20
Hydrophobic TM3 R 156 3x50 G.H5 C 351 G.H5.23
Hydrophobic ICL2 L 167 34x54 G.S3 L 194 G.S3.01
Hydrophobic ICL2 P 163 34x50 G.H5 T 340 G.H5.12
Van-der-waals ICL2 P 163 34x50 G.H5 T 340 G.H5.12
Hydrophobic TM6 I 272 6x33 G.H5 L 348 G.H5.20
Hydrophobic TM3 A 159 3x53 G.H5 C 351 G.H5.23
Hydrophobic TM5 M 249 5x61 G.H5 L 353 G.H5.25
Van-der-waals TM5 M 249 5x61 G.H5 L 353 G.H5.25
Hydrophobic TM3 V 160 3x54 G.H5 L 348 G.H5.20
Hydrophobic ICL2 V 164 34x51 G.H5 F 336 G.H5.08
Hydrophobic ICL2 P 163 34x50 G.H5 I 344 G.H5.16
Van-der-waals ICL2 P 163 34x50 G.H5 I 344 G.H5.16
Hydrophobic H8 D 334 8x47 G.H5 G 352 G.H5.24
Van-der-waals H8 D 334 8x47 G.H5 G 352 G.H5.24
Hydrophobic TM6 I 272 6x33 G.H5 L 353 G.H5.25
Van-der-waals TM6 I 272 6x33 G.H5 L 353 G.H5.25
Hydrophobic TM3 V 160 3x54 G.H5 I 344 G.H5.16
Hydrophobic ICL3 L 258 - G.H5 F 354 G.H5.26
Polar TM6 K 265 6x26 G.h4s6 K 314 G.h4s6.08
Hydrophobic ICL3 L 258 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 L 258 - G.h4s6 E 318 G.h4s6.12
Polar ICL3 R 257 - G.S6 I 319 G.S6.01
Hydrophobic ICL3 R 257 - G.S6 I 319 G.S6.01
Van-der-waals ICL3 R 257 - G.S6 I 319 G.S6.01
Hydrophobic TM5 L 253 5x65 G.H5 L 348 G.H5.20
Van-der-waals TM5 L 253 5x65 G.H5 L 348 G.H5.20
Aromatic ICL3 R 257 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 R 257 - G.S6 Y 320 G.S6.02
Polar ICL2 R 170 34x57 G.H5 C 351 G.H5.23
Hydrophobic ICL2 R 170 34x57 G.H5 C 351 G.H5.23
Van-der-waals ICL2 R 170 34x57 G.H5 C 351 G.H5.23
Hydrophobic ICL3 V 256 - G.H5 I 344 G.H5.16
Hydrophobic TM3 R 156 3x50 G.H5 L 353 G.H5.25
Van-der-waals TM3 R 156 3x50 G.H5 L 353 G.H5.25
Hydrophobic ICL2 L 167 34x54 G.hns1 R 32 G.hns1.03
Ionic TM6 K 265 6x26 G.h4s6 E 318 G.h4s6.12
Polar TM6 K 265 6x26 G.h4s6 E 318 G.h4s6.12
Van-der-waals TM6 K 265 6x26 G.h4s6 E 318 G.h4s6.12
Polar TM3 A 159 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 A 159 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 A 159 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL3 L 258 - G.H5 K 345 G.H5.17
Polar TM6 R 271 6x32 G.H5 L 353 G.H5.25
Ionic ICL3 R 257 - G.h4s6 E 318 G.h4s6.12
Polar ICL3 R 257 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 R 257 - G.h4s6 E 318 G.h4s6.12