GPR4 - Gs

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Xenopus tropicalis

GPCR PREFERRED CHAIN

R

G PROTEIN (FAMILY)

Gs

G PROTEIN SPECIES

Homo sapiens

PDB CODE

8ZF6

RESOLUTION

3.0 Å

REFERENCE

10.1016/J.CELL.2024.12.001

PUBLICATION DATE

Feb. 26, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

0 interactions (Click to see)

LIGANDS

Apo (no ligand) -

PHYSIOLOGICAL LIGANDS

No physiological ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar TM3 A 120 3x55 G.H5 R 380 G.H5.12
Van-der-waals TM3 A 120 3x55 G.H5 R 380 G.H5.12
Polar ICL3 A 213 - G.h4s6 Y 358 G.h4s6.20
Hydrophobic ICL3 A 213 - G.h4s6 Y 358 G.h4s6.20
Van-der-waals ICL3 A 213 - G.h4s6 Y 358 G.h4s6.20
Polar ICL3 S 214 - G.H5 R 385 G.H5.17
Hydrophobic ICL3 S 214 - G.H5 R 385 G.H5.17
Van-der-waals ICL3 S 214 - G.H5 R 385 G.H5.17
Polar ICL3 S 214 - G.H5 D 381 G.H5.13
Hydrophobic ICL3 S 214 - G.H5 D 381 G.H5.13
Hydrophobic ICL2 L 123 - G.S1 H 41 G.S1.02
Van-der-waals ICL2 L 123 - G.S1 H 41 G.S1.02
Hydrophobic TM3 A 118 3x53 G.H5 Y 391 G.H5.23
Van-der-waals TM3 A 118 3x53 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 R 124 - G.S3 V 227 G.S3.03
Van-der-waals ICL2 R 124 - G.S3 V 227 G.S3.03
Hydrophobic TM6 V 223 6x33 G.H5 L 393 G.H5.25
Van-der-waals TM1 Q 45 1x60 G.H5 E 392 G.H5.24
Hydrophobic ICL2 L 123 - G.H5 R 380 G.H5.12
Van-der-waals ICL2 L 123 - G.H5 R 380 G.H5.12
Hydrophobic TM5 L 209 5x65 G.H5 L 388 G.H5.20
Polar ICL1 E 51 - G.H5 Y 391 G.H5.23
Hydrophobic ICL1 E 51 - G.H5 Y 391 G.H5.23
Van-der-waals ICL1 E 51 - G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 115 3x50 G.H5 L 393 G.H5.25
Hydrophobic TM5 Y 202 5x58 G.H5 L 393 G.H5.25
Polar ICL2 P 122 - G.H5 H 387 G.H5.19
Hydrophobic ICL2 P 122 - G.H5 H 387 G.H5.19
Van-der-waals ICL2 P 122 - G.H5 H 387 G.H5.19
Hydrophobic TM3 V 119 3x54 G.H5 L 393 G.H5.25
Hydrophobic ICL2 P 122 - G.H5 Q 384 G.H5.16
Van-der-waals ICL2 P 122 - G.H5 Q 384 G.H5.16
Hydrophobic ICL2 L 123 - G.S3 V 227 G.S3.03
Polar ICL1 R 50 - G.H5 Q 390 G.H5.22
Hydrophobic ICL1 R 50 - G.H5 Q 390 G.H5.22
Polar TM4 R 129 4x37 G.HN Q 31 G.HN.48
Hydrophobic TM4 R 129 4x37 G.HN Q 31 G.HN.48
Van-der-waals TM4 R 129 4x37 G.HN Q 31 G.HN.48
Polar ICL3 S 214 - G.S6 Y 360 G.S6.02
Van-der-waals ICL3 S 214 - G.S6 Y 360 G.S6.02
Hydrophobic TM3 V 119 3x54 G.H5 L 388 G.H5.20
Van-der-waals TM3 V 119 3x54 G.H5 L 388 G.H5.20
Hydrophobic TM6 V 223 6x33 G.H5 L 394 G.H5.26
Van-der-waals TM6 V 223 6x33 G.H5 L 394 G.H5.26
Hydrophobic TM6 L 226 6x36 G.H5 L 393 G.H5.25
Van-der-waals TM6 L 226 6x36 G.H5 L 393 G.H5.25
Polar ICL2 F 125 - G.H5 H 387 G.H5.19
Van-der-waals ICL2 F 125 - G.H5 H 387 G.H5.19
Polar TM3 V 119 3x54 G.H5 Q 384 G.H5.16
Hydrophobic TM3 V 119 3x54 G.H5 Q 384 G.H5.16
Van-der-waals TM3 V 119 3x54 G.H5 Q 384 G.H5.16
Polar TM3 D 114 3x49 G.H5 Y 391 G.H5.23
Hydrophobic TM3 D 114 3x49 G.H5 Y 391 G.H5.23
Van-der-waals TM3 D 114 3x49 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 P 122 - G.H5 I 383 G.H5.15
Van-der-waals ICL2 P 122 - G.H5 I 383 G.H5.15
Polar TM3 R 115 3x50 G.H5 Y 391 G.H5.23
Hydrophobic TM3 R 115 3x50 G.H5 Y 391 G.H5.23
Van-der-waals TM3 R 115 3x50 G.H5 Y 391 G.H5.23
Hydrophobic ICL2 L 123 - G.H5 I 383 G.H5.15
Polar TM3 R 115 3x50 G.H5 E 392 G.H5.24
Van-der-waals TM3 R 115 3x50 G.H5 E 392 G.H5.24
Hydrophobic ICL2 P 122 - G.H5 R 380 G.H5.12
Polar ICL2 R 124 - G.S3 D 225 G.S3.01
Van-der-waals ICL2 R 124 - G.S3 D 225 G.S3.01
Hydrophobic ICL3 S 214 - G.h4s6 Y 358 G.h4s6.20
Polar ICL3 A 213 - G.H5 D 381 G.H5.13
Hydrophobic ICL2 L 123 - G.H5 F 376 G.H5.08
Van-der-waals ICL2 L 123 - G.H5 F 376 G.H5.08
Hydrophobic ICL3 C 215 - G.H5 L 388 G.H5.20
Hydrophobic TM2 L 52 2x39 G.H5 Y 391 G.H5.23
Van-der-waals TM2 L 52 2x39 G.H5 Y 391 G.H5.23
Hydrophobic ICL3 S 212 - G.H5 D 381 G.H5.13
Polar TM5 A 208 5x64 G.H5 Q 384 G.H5.16
Van-der-waals TM5 A 208 5x64 G.H5 Q 384 G.H5.16
Polar TM3 A 118 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM3 A 118 3x53 G.H5 H 387 G.H5.19
Van-der-waals TM3 A 118 3x53 G.H5 H 387 G.H5.19
Hydrophobic TM5 L 205 5x61 G.H5 L 393 G.H5.25
Van-der-waals TM5 L 205 5x61 G.H5 L 393 G.H5.25