ORF74 - Gi1

GPCR CLASS (FAMILY)

Class A (Rhodopsin)

GPCR SPECIES

Human herpesvirus 8 type P

GPCR PREFERRED CHAIN

E

G PROTEIN (FAMILY)

Gi/o

G PROTEIN SPECIES

Homo sapiens

PDB CODE

9EJC

RESOLUTION

3.0 Å

REFERENCE

10.1038/S41467-025-63457-4

PUBLICATION DATE

Sept. 3, 2025

3D VIEW

NGL is a WebGL based 3D viewer powered by MMTF.

LIGAND INTERACTIONS

75 interactions (Click to see)

LIGANDS

CXCL1 - Agonist

PHYSIOLOGICAL LIGANDS

No physiological ligands available



GPCR - G protein interface interactions



The biflare plot is a modified version of a flareplot, where lines are drawn between an inner concentric circle (Gα residues) and an outer concentric circle (GPCR residues) depicting the interface interactions.
Different interaction types are shown with different line types (aromatic - solid, hydrophobic - dashed, ionic - long dashed, polar - dotted, Van der Waals - dash-dot).
The interactive features include filtering on interaction type, segments, residues and backbone or sidechain interactions.


Interaction definitions:

Residue numbering:

GPCR-G protein backbone (bb) or sidechain (sc):





Structures

Sequence Color

Gα, Gβ, Gγ, GPCR
Interaction cut-off: strict (stick), loose (line)

NGL is a WebGL based 3D viewer powered by MMTF.

Interaction
type		 GPCR
Segment
AA
Number		 Generic
number		 Gα
segment
AA
Number		 Generic
number
Polar ICL3 Q 244 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 Q 244 - G.h4s6 E 318 G.h4s6.12
Hydrophobic TM6 V 249 6x33 G.H5 F 354 G.H5.26
Hydrophobic ICL3 L 243 - G.H5 K 345 G.H5.17
Van-der-waals ICL3 L 243 - G.H5 K 345 G.H5.17
Hydrophobic TM6 V 249 6x33 G.H5 L 348 G.H5.20
Polar ICL2 S 150 - G.H5 N 347 G.H5.19
Hydrophobic ICL2 S 150 - G.H5 N 347 G.H5.19
Van-der-waals ICL2 S 150 - G.H5 N 347 G.H5.19
Van-der-waals ICL3 L 243 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 L 243 - G.H5 D 341 G.H5.13
Hydrophobic ICL2 S 150 - G.H5 I 343 G.H5.15
Polar TM3 R 143 3x50 G.H5 G 352 G.H5.24
Hydrophobic TM3 R 143 3x50 G.H5 G 352 G.H5.24
Van-der-waals TM3 R 143 3x50 G.H5 G 352 G.H5.24
Polar ICL3 Q 244 - G.h4s6 D 315 G.h4s6.09
Hydrophobic ICL3 Q 244 - G.h4s6 D 315 G.h4s6.09
Van-der-waals ICL3 Q 244 - G.h4s6 D 315 G.h4s6.09
Polar TM6 K 248 6x32 G.H5 F 354 G.H5.26
Van-der-waals TM6 K 248 6x32 G.H5 F 354 G.H5.26
Hydrophobic ICL3 T 241 - G.H5 I 344 G.H5.16
Van-der-waals ICL3 T 241 - G.H5 I 344 G.H5.16
Ionic ICL2 R 152 - G.s2s3 D 193 G.s2s3.02
Polar ICL2 R 152 - G.s2s3 D 193 G.s2s3.02
Van-der-waals ICL2 R 152 - G.s2s3 D 193 G.s2s3.02
Polar ICL2 S 153 - G.HN E 28 G.HN.52
Hydrophobic TM3 V 147 3x54 G.H5 I 344 G.H5.16
Aromatic TM3 R 143 3x50 G.H5 F 354 G.H5.26
Hydrophobic TM3 R 143 3x50 G.H5 F 354 G.H5.26
Van-der-waals TM3 R 143 3x50 G.H5 F 354 G.H5.26
Polar ICL2 S 153 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 S 153 - G.hns1 R 32 G.hns1.03
Hydrophobic TM2 I 82 2x39 G.H5 L 353 G.H5.25
Van-der-waals TM2 I 82 2x39 G.H5 L 353 G.H5.25
Hydrophobic TM6 V 252 6x36 G.H5 F 354 G.H5.26
Van-der-waals TM6 V 252 6x36 G.H5 F 354 G.H5.26
Polar ICL2 K 156 - G.H5 N 347 G.H5.19
Hydrophobic ICL3 L 243 - G.h4s6 E 318 G.h4s6.12
Van-der-waals ICL3 L 243 - G.h4s6 E 318 G.h4s6.12
Polar ICL2 T 151 - G.s2s3 D 193 G.s2s3.02
Hydrophobic ICL2 K 156 - G.H5 C 351 G.H5.23
Van-der-waals ICL2 K 156 - G.H5 C 351 G.H5.23
Hydrophobic TM3 L 146 3x53 G.H5 G 352 G.H5.24
Hydrophobic ICL2 T 151 - G.hns1 R 32 G.hns1.03
Van-der-waals ICL2 T 151 - G.hns1 R 32 G.hns1.03
Polar TM3 L 146 3x53 G.H5 N 347 G.H5.19
Hydrophobic TM3 L 146 3x53 G.H5 N 347 G.H5.19
Van-der-waals TM3 L 146 3x53 G.H5 N 347 G.H5.19
Hydrophobic ICL2 R 152 - G.hns1 R 32 G.hns1.03
Hydrophobic TM3 L 146 3x53 G.H5 L 348 G.H5.20
Polar ICL3 K 242 - G.S6 Y 320 G.S6.02
Hydrophobic ICL3 K 242 - G.S6 Y 320 G.S6.02
Van-der-waals ICL3 K 242 - G.S6 Y 320 G.S6.02
Van-der-waals H8 S 319 8x48 G.H5 F 354 G.H5.26
Polar ICL3 Q 244 - G.h4s6 T 316 G.h4s6.10
Hydrophobic TM3 L 146 3x53 G.H5 C 351 G.H5.23
Hydrophobic TM2 L 86 2x43 G.H5 L 353 G.H5.25
Van-der-waals TM2 L 86 2x43 G.H5 L 353 G.H5.25
Hydrophobic TM6 I 253 6x37 G.H5 F 354 G.H5.26
Polar TM7 G 318 7x57 G.H5 L 353 G.H5.25
Hydrophobic TM7 G 318 7x57 G.H5 L 353 G.H5.25
Van-der-waals TM7 G 318 7x57 G.H5 L 353 G.H5.25
Hydrophobic ICL3 T 241 - G.H5 D 341 G.H5.13
Van-der-waals ICL3 T 241 - G.H5 D 341 G.H5.13
Polar ICL3 Q 244 - G.h4s6 K 314 G.h4s6.08
Hydrophobic ICL3 Q 244 - G.h4s6 K 314 G.h4s6.08
Van-der-waals ICL3 Q 244 - G.h4s6 K 314 G.h4s6.08
Hydrophobic TM7 G 318 7x57 G.H5 F 354 G.H5.26
Ionic ICL2 K 157 - G.HN E 28 G.HN.52
Polar ICL2 K 157 - G.HN E 28 G.HN.52
Hydrophobic ICL2 K 157 - G.HN E 28 G.HN.52
Van-der-waals ICL2 K 157 - G.HN E 28 G.HN.52
Hydrophobic TM5 V 238 5x65 G.H5 L 348 G.H5.20
Hydrophobic TM3 V 147 3x54 G.H5 L 348 G.H5.20
Van-der-waals TM3 V 147 3x54 G.H5 L 348 G.H5.20